In vitro antifungal potential of extracts of stinging nettle (Urtica dioica L.) and rue (Ruta graveolens L.) in inhibiting the growth and sporulation of cocoa moniliasis (Moniliophthora roreri)

Authors

  • Carlos Enrique Torrejon-Tafur Facultad de Ingeniería y Ciencias Agrarias (FICA), Universidad Nacional Toribio Rodríguez de Mendoza de Amazonas, 342 Higos Urco, Chachapoyas 01001, Perú.
  • Santos Triunfo Leiva Espinoza Facultad de Ingeniería y Ciencias Agrarias (FICA): Grupo de investigación en procesos en sanidad vegetal, Universidad Nacional Toribio Rodríguez de Mendoza de Amazonas, 342 Higos Urco, Chachapoyas 01001, Perú. https://orcid.org/0000-0003-1710-1994

DOI:

https://doi.org/10.25127/agrops.20251.1151

Keywords:

Moniliophthora roreri, antifungal, plant extracts, Urtica dioica, Ruta graveolens

Abstract

The objective of this study was to evaluate the in vitro effect of extracts and essential oils from stinging nettle (Urtica dioica L.) and rue (Ruta graveolens L.) on Moniliophthora roreri, the causal agent of “cocoa moniliasis.” The essential oils were obtained by steam distillation, and the extracts by maceration. To evaluate the variables of radial growth inhibition and sporulation, a completely randomized design (CRD) was used in a PDA culture medium incubated at 28 °C, with five treatments of five replicates, including a control. Each treatment evaluated five concentrations (0%, 5%, 10%, 15%, and 20% v/v). An analysis of variance (ANOVA) at the 5% level and a Tukey multiple-comparison test (α = 0.05) were performed to identify specific differences between means. The results showed that 20% nettle extracts inhibited radial growth by 53.78% and sporulation by 77.95%, while 20% rue extracts inhibited radial growth by 47.28% and sporulation by 79.37%. It is concluded that nettle and rue extracts have the potential to control moniliasis in cocoa.

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Published

2025-12-11

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How to Cite

In vitro antifungal potential of extracts of stinging nettle (Urtica dioica L.) and rue (Ruta graveolens L.) in inhibiting the growth and sporulation of cocoa moniliasis (Moniliophthora roreri). (2025). Revista De Investigación De Agroproducción Sustentable, 7(1), 116-128. https://doi.org/10.25127/agrops.20251.1151

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